pG106 vector (V006138)

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LOCUS       V006138                 9278 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006138
VERSION     V006138
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Jones KR, Macrina FL, Lewis JP.
  TITLE     Porphyromonas gingivalis shuttle vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9278)
  AUTHORS   Jones KR, Macrina FL, Lewis JP.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2017) OCMB, Virginia Commonwealth University,
            Philips Institute, 521 North 11th Street, Richmond, VA 23298, USA
REFERENCE   3  (bases 1 to 9278)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9278)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-JUL-2017) OCMB, Virginia Commonwealth University, Philips
            Institute, 521 North 11th Street, Richmond, VA 23298, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             316..324
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             767..2359
                     /codon_start=1
                     /gene="repB"
                     /product="RepB"
                     /label="repB"
                     /note="putative protein required for plasmid replication in
                     Porphyromonas gingivalis"
                     /protein_id="AVN57617.1"
                     /translation="MRTFEYRSLKPFEFFQIIFGSCDHNDPQCPIYPFEKELCQEYIDW
                     RAQQAGRKSKSKAGKKAIYNPAQPFRYNALVQAVYSVEPDAGRANDNGRRSKTWVVVGD
                     ATSEDDIKVCGWDSLEELMGRYKFVVTTPATYVGRRRFKSNARYLYALAFDLDEVGVPE
                     MSEVIHQQTIGMTPQANIIVNSGDGIHLYYILAKPMPLYPKVYETLTNIKKALTRLIWN
                     KASSRTGEDNQVQIQPIIQPFRVPGSRTKHGDIVTAWHNADAPLHTIEELNRFASKPTL
                     KYLNSGVSQEQAQAFGQRGILPRIWLTKARAKELYPDWYQKRIVEGAPRKTWHVHRGLY
                     EWWLHMLWTNEKVAVGHRYHCIMFLAVYAKKCNVPFDILKRDALELVPRMEALTGNTGR
                     HFSVQDALDALKAYKESSETYPRQLIEDRSGLRIEPNKRNGRPQDLHLKLARGSRDILQ
                     EAKGRKDWREGNGRPKGSIVLAEDSPQYAKVQEWRDRNPNSNNKSRCARETGLSRPTRP
                     QVVAKPLGNALRT"
     gene            767..2359
                     /gene="repB"
                     /label="repB"
     CDS             complement(2518..3495)
                     /codon_start=1
                     /gene="mob"
                     /product="Mob"
                     /label="mob"
                     /note="putative protein required for plasmid stability in
                     Porphyromonas gingivalis"
                     /protein_id="AVN57618.1"
                     /translation="MSKTSIHIEPCKIGSSEQHNQRLKHLDYVRPDLSPQNESWVGVAD
                     LPAHLEQLRILVKEKTGRAMQQKATPIREGVIVINQGTSINQLRGLATAIELRWGIKTL
                     QIYTHKDEGHIDSDDTWKPNLHAHMVLDWVNHDTGKSIKLSKQDMAEMQTMVADCLQMV
                     RGESSDVKHLGAIQYKNQAEELRLQTLKEQTMKEEQARELALAEAKRMEQVASEQIIAE
                     NKKLGGLWMDWQKSFEASEKRRESITARKIEEDKLWEELGLKGAFKSFWEECKDWFRGF
                     GDGLKDLALLIWASPRGVMDKDVRCTADRKRGKLLLKWAYTRSR"
     gene            complement(2518..3495)
                     /gene="mob"
                     /label="mob"
     CDS             complement(4510..5043)
                     /codon_start=1
                     /product="hypothetical protein"
                     /label="hypothetical protein"
                     /note="orfE; hypothetical protein"
                     /protein_id="AVN57619.1"
                     /translation="MLVRGAEPMEKRQQRGLFTVPGLLLAFCSHGSSIADGLVAHPSEG
                     FYIIYTTSSEEFLTLSEEDYSLFASFANLVVREDSNSNGLRSAKPSGDGWKFGGKGKGK
                     LDAIHIGYELSKILGENQDFEIYVEYNGDGSYTVWYREVQKSSKGSNPVKTFNVLIRAN
                     EREGHTVSLFQPYA"
     rep_origin      complement(5013..5601)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5942..6676)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     CDS             complement(6912..7712)
                     /codon_start=1
                     /gene="ermF"
                     /product="ErmF"
                     /label="ermF"
                     /note="erythromycin resistance in Bacteroidetes"
                     /protein_id="AVN57621.1"
                     /translation="MTKKKLPVRFTGQHFTIDKVLIKDAIRQANISNQDTVLDIGAGKG
                     FLTVHLLKIANNVVAIENDTALVEHLRKLFSDARNVQVVGCDFRNFAVPKFPFKVVSNI
                     PYGITSDIFKILMFESLGNFLGGSIVLQLEPTQKLFSRKLYNPYTVFYHTFFDLKLVYE
                     VGPESFLPPPTVKSALLNIKRKHLFFDFKFKAKYLAFISCLLEKPDLSVKTALKSIFRK
                     SQVRSISEKFGLNLNAQIVCLSPSQWLNCFLEMLEVVPEKFHPS"
     gene            complement(6912..7712)
                     /gene="ermF"
                     /label="ermF"
     oriT            8269..8378
                     /label="oriT"
                     /note="incP origin of transfer"
     primer_bind     8847..8863
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    8864..8920
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8933..8949)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(8957..8973)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8981..9011)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9026..9047)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."