pFYTAG vector (V006143)

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LOCUS       40924_20786       11166 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway adapted binary vector pFYTAG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11166)
  AUTHORS   Zhang C, Galbraith DW.
  TITLE     A Gateway adapted Agrobacterium binary vector series for plant 
            biological studies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11166)
  AUTHORS   Zhang C, Galbraith DW.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2006) Department of Plant Sciences, University of 
            Arizona, 303 Forbes Building, Tucson, AZ 85721-0036, USA
REFERENCE   3  (bases 1 to 11166)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11166)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-JAN-2006) Department of Plant Sciences, University of Arizona, 
            303 Forbes Building, Tucson, AZ 85721-0036, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             425..1216
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1306..1894
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2080..2220)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2564..2758)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2827..3891)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4328..4954)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    complement(6254..6278)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     6481..6497
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6523..6646
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        6672..6702
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             6756..7433
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             7756..8058
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(8102..8226)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     5'UTR           8232..8366
                     /note="translation leader sequence; 5'UTR from tobacco etch
                     virus"
     CDS             8821..9537
                     /label=EYFP
                     /note="enhanced YFP"
     misc_feature    9551..9620
                     /note="computer generated random sequence as a tag of the 
                     HTA6-EYFP fusion transcript"
     regulatory      9625..9835
                     /label=cauliflower mosaic virus 35S poly(A) signal
                     /note="cauliflower mosaic virus 35S poly(A) signal"
                     /regulatory_class="polyA_signal_sequence"
     regulatory      9857..10237
                     /label=mannopine synthase promoter
                     /note="mannopine synthase promoter"
                     /regulatory_class="promoter"
     promoter        9857..10237
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             10243..10791
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     terminator      10804..11056
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    complement(11142..11166)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"