pFYS028 vector (V006144)

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LOCUS       40924_20781        4675 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFYS028, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Scott FY, Ruder WC.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2016) Biological Systems Engineering, Virginia 
            Polytechnic Institute and State University, 1230 Washington Street 
            SW, Blacksburg, VA 24061, USA
REFERENCE   2  (bases 1 to 4675)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4675)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (21-SEP-2016) Biological Systems Engineering, Virginia Polytechnic 
            Institute and State University, 1230 Washington Street SW, 
            Blacksburg, VA 24061, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      21..115
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(139..846)
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     promoter        complement(866..1150)
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     promoter        1241..1525
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     regulatory      1532..1542
                     /regulatory_class="ribosome_binding_site"
     CDS             1990..2535
                     /label=SNAP-tag(R)
                     /note="human O6-alkylguanine-DNA-alkyltransferase variant
                     that forms covalent bonds with benzylguanine derivatives"
     terminator      2635..2721
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2885..3473)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(3561..3655)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3688..4545)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4546..4650)
                     /label=AmpR promoter