pFUN vector (V006154)

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LOCUS       V006154                 8072 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006154
VERSION     V006154
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Poquet I, Ehrlich SD, Gruss A.
  TITLE     An export-specific reporter designed for gram-positive bacteria:
            application to Lactococcus lactis
  JOURNAL   J. Bacteriol. 180 (7), 1904-1912 (1998)
   PUBMED   9537391
REFERENCE   2  (bases 1 to 8072)
  AUTHORS   Poquet I, Gruss A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-1997) Laboratoire de Genetique Appliquee-URLGA,
            Institut National de la Recherche Agronomique, CRJ, Jouy en Josas
            78352, France
REFERENCE   3  (bases 1 to 8072)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8072)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "1998"; volume: "180"; issue: "7"; pages:
            "1904-1912"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-DEC-1997) Laboratoire de Genetique Appliquee-URLGA, Institut
            National de la Recherche Agronomique, CRJ, Jouy en Josas 78352,
            France"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             539..1273
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     promoter        complement(4599..4617)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4624..4640)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      complement(4781..5236)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5263..5367
                     /label="AmpR promoter"
     CDS             5368..6225
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6399..6987
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    7275..7296
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7311..7341
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7349..7365
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7373..7389
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        7410..7428
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     7458..7474
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      7473..7507
                     /label="trpA terminator"
                     /note="trpA terminator"
                     /regulatory_class="terminator"
     misc_feature    7512..7547
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     CDS             7561..8007
                     /label="MNase"
                     /note="micrococcal nuclease from Staphylococcus aureus"