pFU86 vector (V006158)

LOCUS       V006158                 5007 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006158
VERSION     V006158
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Uliczka F, Pisano F, Kochut A, Opitz W, Herbst K, Stolz T, Dersch P.
  TITLE     Monitoring of Gene Expression in Bacteria during Infections Using an
            Adaptable Set of Bioluminescent, Fluorescent and Colorigenic Fusion
            Vectors
  JOURNAL   PLoS ONE 6 (6), E20425 (2011)
   PUBMED   21673990
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Uliczka F.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2011) Department of Molecular Infection Biology,
            Helmholtz Centre for Infection Research, Inhoffenstrasse 7,
            Braunschweig, Niedersachsen 38124, Germany
REFERENCE   3  (bases 1 to 5007)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5007)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2011"; volume: "6"; issue: "6"; pages: "E20425"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-APR-2011) Department of Molecular Infection Biology, Helmholtz
            Centre for Infection Research, Inhoffenstrasse 7, Braunschweig,
            Niedersachsen 38124, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      36..42
                     /gene="phoA"
                     /regulatory_class="ribosome_binding_site"
     CDS             50..1462
                     /gene="phoA"
                     /label="Alkaline phosphatase"
                     /note="Alkaline phosphatase from Escherichia coli (strain
                     K12). Accession#: P00634"
     terminator      1485..1571
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(2109..3056)
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     rep_origin      complement(3104..3326)
                     /direction=LEFT
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3818..3912)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(3945..4802)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4803..4907)
                     /label="AmpR promoter"