pFU54 vector (V006163)

LOCUS       40924_20666        9388 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter gene-fusion vector pFU54, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Uliczka F, Pisano F, Kochut A, Opitz W, Herbst K, Stolz T, Dersch P.
  TITLE     Monitoring of Gene Expression in Bacteria during Infections Using an
            Adaptable Set of Bioluminescent, Fluorescent and Colorigenic Fusion 
            Vectors
  JOURNAL   PLoS ONE 6 (6), E20425 (2011)
  PUBMED    21673990
REFERENCE   2  (bases 1 to 9388)
  AUTHORS   Uliczka F.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2011) Department of Molecular Infection Biology, 
            Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 
            Braunschweig, Niedersachsen 38124, Germany
REFERENCE   3  (bases 1 to 9388)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9388)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2011"; volume: "6"; issue: "6"; pages: "E20425"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-APR-2011) Department of Molecular Infection Biology, Helmholtz 
            Centre for Infection Research, Inhoffenstrasse 7, Braunschweig, 
            Niedersachsen 38124, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      36..42
                     /gene="luxC"
                     /regulatory_class="ribosome_binding_site"
     CDS             48..1487
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
     CDS             1503..2423
                     /label=LuxD
                     /note="LuxD acyltransferase"
     CDS             2475..3554
                     /label=LuxA
                     /note="LuxA luciferase subunit"
     CDS             3572..4552
                     /label=LuxB
                     /note="LuxB luciferase subunit"
     CDS             4734..5843
                     /label=LuxE
                     /note="LuxE"
     terminator      5866..5952
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(6490..7437)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(7485..7707)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(8199..8293)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(8326..9183)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9184..9288)
                     /label=AmpR promoter