pFTMGW vector (V006174)

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LOCUS       V006174                 8928 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006174
VERSION     V006174
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Geller SF, Ge PS, Visel M, Greenberg KP, Flannery JG.
  TITLE     Functional promoter testing using a modified lentiviral transfer
            vector
  JOURNAL   Mol. Vis. 13, 730-739 (2007)
   PUBMED   17563724
REFERENCE   2  (bases 1 to 8928)
  AUTHORS   Geller SF, Ge PS.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2006) Helen Wills Neuroscience Institute,
            University of California, Berkeley, 114 Barker Hall, Berkeley, CA
            94720-3190, USA
REFERENCE   3  (bases 1 to 8928)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8928)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Vis.
            13, 730-739 (2007)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-DEC-2006) Helen Wills Neuroscience Institute, University of
            California, Berkeley, 114 Barker Hall, Berkeley, CA 94720-3190, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..820
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2098..2142
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2291..2332
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2440..2557
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     polyA_signal    2608..2656
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    2670..2761
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     misc_feature    2767..2835
                     /note="MCS; Multiple Cloning Site"
     CDS             2860..3576
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    3620..4208
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(4211..4227)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     repeat_region   4413..4914
                     /label="3' LTR from lentivirus"
                     /note="3' LTR from lentivirus"
     LTR             4733..4913
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    4945..5169
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5215..5643
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5657..5986
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        6034..6081
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             6100..6471
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    6604..6737
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6774..6790)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6798..6814)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6822..6852)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6867..6888)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7176..7764)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7938..8795)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8796..8900)
                     /label="AmpR promoter"