pFTM3GW vector (V006175)

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LOCUS       V006175                 8956 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006175
VERSION     V006175
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8956)
  AUTHORS   Geller SF, Ge PS, Visel M, Flannery JG.
  TITLE     In vitro analysis of promoter activity in Muller cells
  JOURNAL   Mol. Vis. 14, 691-705 (2008)
   PUBMED   18437242
REFERENCE   2  (bases 1 to 8956)
  AUTHORS   Geller SF, Ge PS, Visel M, Flannery JG.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2009) Helen Wills Neuroscience Institute,
            University of California, Berkeley, 114 Barker Hall, Berkeley, CA
            94720, USA
REFERENCE   3  (bases 1 to 8956)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8956)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Vis.
            14, 691-705 (2008)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-MAR-2009) Helen Wills Neuroscience Institute, University of
            California, Berkeley, 114 Barker Hall, Berkeley, CA 94720, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..623
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        624..826
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             841..1021
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1074..1199
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1692..1925
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2110..2154
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2303..2344
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2452..2569
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     polyA_signal    2620..2668
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    2682..2773
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     misc_feature    2789..2863
                     /label="MCS - multiple cloning site"
                     /note="MCS - multiple cloning site"
     CDS             2888..3604
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    3648..4236
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(4239..4255)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     repeat_region   4441..4942
                     /label="3' LTR - Lentiviral long terminal repeat"
                     /note="3' LTR - Lentiviral long terminal repeat"
     LTR             4761..4941
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    4973..5197
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5243..5671
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5685..6014
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        6062..6109
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             6128..6499
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    6632..6765
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6802..6818)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6826..6842)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6850..6880)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6895..6916)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7204..7792)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7966..8823)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8824..8928)
                     /label="AmpR promoter"