Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V007475 | pAUR123 | In stock, instant shipping |
Buy one, get one free! |
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.
Basic Vector Information
- Vector Name:
- pAUR123
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6982 bp
- Type:
- Yeast Expression Vectors
- Replication origin:
- ori
- Promoter:
- ADH1
- Cloning Method:
- Enzyme digestion and ligation
- Growth Strain(s):
- DH10B
- Growth Temperature:
- 37℃
pAUR123 vector Map
Plasmid Protocol
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5. Store the plasmid at -20 ℃.
6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it
General Plasmid Transform Protocol
1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.
2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.
3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.
4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.
5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.
pAUR123 vector Sequence
LOCUS V007475 6982 bp DNA circular SYN 13-JAN-2022
DEFINITION Exported.
ACCESSION V007475
VERSION V007475
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 6982)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 6982)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6982
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 152..256
/label="AmpR promoter"
CDS 257..1114
/label="AmpR"
/note="beta-lactamase"
rep_origin 1288..1876
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(2062..2202)
/label="bom"
/note="basis of mobility region from pBR322"
misc_feature complement(2903..3195)
/label="CEN4"
/note="S. cerevisiae CEN4 centromere"
rep_origin complement(3196..3649)
/direction=LEFT
/label="ARS1"
/note="S. cerevisiae autonomously replicating sequence
ARS1"
CDS 4017..5219
/label="AurR"
/note="aureobasidin A-resistant mutant of S. cerevisiae
inositol phosphorylceramide synthase (Aeed et al., 2009)"
CDS complement(5537..5929)
/gene="MRP17"
/label="Small ribosomal subunit protein bS6m"
/note="Small ribosomal subunit protein bS6m from
Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
Accession#: P28778"
promoter 6190..6590
/label="ADH1 promoter"
/note="promoter for alcohol dehydrogenase 1"
terminator 6628..6815
/label="ADH1 terminator"
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"