Basic Vector Information
- Vector Name:
- pEnEOi9mycThsp
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4715 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Koiwa H.
- Promoter:
- CaMV35S(enhanced)
pEnEOi9mycThsp vector Map
pEnEOi9mycThsp vector Sequence
LOCUS 40924_17414 4715 bp DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector pEnEOi9mycThsp, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4715)
AUTHORS Koiwa H.
TITLE Arabidopsis thaliana CPL4 is an essential CTD-phosphatase
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4715)
AUTHORS Koiwa H.
TITLE Direct Submission
JOURNAL Submitted (14-AUG-2013) Department of Horticultural Science, Texas A
REFERENCE 3 (bases 1 to 4715)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4715)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(14-AUG-2013) Department of Horticultural Science, Texas A"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..4715
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(63..651)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(744..1550)
/label=KanR
/note="aminoglycoside phosphotransferase"
protein_bind 1673..1772
/label=attL2
/note="recombination site for the Gateway(R) LR reaction"
promoter 1884..2551
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
regulatory 2555..2625
/label=derived from Tobacco Mosaic Virus Omega sequence
/note="derived from Tobacco Mosaic Virus Omega sequence"
/regulatory_class="other"
intron 2646..3240
/note="derived from EF1a"
CDS 3307..3336
/label=Myc
/note="Myc (human c-Myc proto-oncogene) epitope tag"
CDS 3343..3372
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3379..3408
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3427..3456
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3463..3492
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3499..3528
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3547..3576
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3583..3612
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3619..3648
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
terminator 3713..3961
/label=HSP terminator
/note="efficient transcription terminator from the
Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya
et al., 2010)"
terminator 3986..4238
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
protein_bind complement(4259..4358)
/label=attL1
/note="recombination site for the Gateway(R) LR reaction"
terminator complement(4408..4435)
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator complement(4527..4613)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
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