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pEMY07CD_Ttdh1_TER38 vector (V007568)

Basic Vector Information

Vector Name:
pEMY07CD_Ttdh1_TER38
Antibiotic Resistance:
Ampicillin
Length:
3174 bp
Type:
Cloning vector
Replication origin:
ori
Source/Author:
Young EM, Zhao Z, Gielesen BE, Wu L, Benjamin Gordon D, Roubos JA, Voigt CA.

pEMY07CD_Ttdh1_TER38 vector Vector Map

pEMY07CD_Ttdh1_TER383174 bp6001200180024003000M13 fwdAmpR promoterAmpRori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pEMY07CD_Ttdh1_TER38 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_17389        3174 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEMY07CD_Ttdh1_TER38, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Young EM, Zhao Z, Gielesen BE, Wu L, Benjamin Gordon D, Roubos JA, 
            Voigt CA.
  TITLE     Iterative algorithm-guided design of massive strain libraries, 
            applied to itaconic acid production in yeast
  JOURNAL   Metab. Eng. 48, 33-43 (2018)
  PUBMED    29753070
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Young E, Zhao Z, Gielesen B, Wu L, Gordon DB, Roubos J, Voigt C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2018) Genetics, DSM Biotechnology Center, 
            Alexander Fleminglaan 1, Delft, South Holland 2613AX, Netherlands
REFERENCE   3  (bases 1 to 3174)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3174)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab. Eng.
            48, 33-43 (2018)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-MAY-2018) Genetics, DSM Biotechnology Center, Alexander 
            Fleminglaan 1, Delft, South Holland 2613AX, Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     790..806
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2030..2121
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2122..2979
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3106..3174
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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