Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V007574 | pEMBL19- | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
- Vector Name:
- pEMBL19-
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3960 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Okamoto S, Niki H.
pEMBL19- vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pEMBL19- vector Sequence
LOCUS 40924_17364 3960 bp DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector pEMBL19- DNA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3960)
AUTHORS Okamoto S, Niki H.
TITLE NBRP cloning vector collection sequence project
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3960)
AUTHORS Okamoto S, Niki H.
TITLE Direct Submission
JOURNAL Submitted (07-APR-2017) Contact:Sho Okamoto National Institute of
Genetics, Microbial Genetics Laboratory; 1111 Yata, Mishima,
Shizuoka 411-8540, Japan URL
:https://www.nig.ac.jp/labs/MicroGen/index.html
REFERENCE 3 (bases 1 to 3960)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3960)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(07-APR-2017) Contact:Sho Okamoto National Institute of Genetics,
Microbial Genetics Laboratory; 1111 Yata, Mishima, Shizuoka
411-8540, Japan URL :https://www.nig.ac.jp/labs/MicroGen/index.html"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3960
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 104..125
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 140..170
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 178..194
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 202..218
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_feature complement(231..287)
/label=MCS
/note="pUC18/19 multiple cloning site"
primer_bind complement(288..304)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(860..1373)
/direction=LEFT
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2052..2156
/label=AmpR promoter
CDS 2157..3014
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3188..3776
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"