Basic Vector Information
- Vector Name:
- pEMBL18 plus
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3960 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Okamoto S, Niki H.
pEMBL18 plus vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pEMBL18 plus vector Sequence
LOCUS 40924_17354 3960 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector pEMBL18 plus DNA, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3960) AUTHORS Okamoto S, Niki H. TITLE NBRP cloning vector collection sequence project JOURNAL Unpublished REFERENCE 2 (bases 1 to 3960) AUTHORS Okamoto S, Niki H. TITLE Direct Submission JOURNAL Submitted (07-APR-2017) Contact:Sho Okamoto National Institute of Genetics, Microbial Genetics Laboratory; 1111 Yata, Mishima, Shizuoka 411-8540, Japan URL :https://www.nig.ac.jp/labs/MicroGen/index.html REFERENCE 3 (bases 1 to 3960) TITLE Direct Submission REFERENCE 4 (bases 1 to 3960) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (07-APR-2017) Contact:Sho Okamoto National Institute of Genetics, Microbial Genetics Laboratory; 1111 Yata, Mishima, Shizuoka 411-8540, Japan URL :https://www.nig.ac.jp/labs/MicroGen/index.html" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..3960 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind 104..125 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 140..170 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 178..194 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 202..218 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" misc_feature 228..284 /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(288..304) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 792..1305 /label=M13 ori /note="M13 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2052..2156 /label=AmpR promoter CDS 2157..3014 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 3188..3776 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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