Basic Vector Information
- Vector Name:
- pELS200
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7828 bp
- Type:
- Shuttle vector
- Replication origin:
- oriV
- Source/Author:
- Diep DB, Johnsborg O, Risoen PA, Nes IF.
pELS200 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pELS200 vector Sequence
LOCUS 40924_17344 7828 bp DNA circular SYN 17-DEC-2018
DEFINITION Shuttle vector pELS200, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7828)
AUTHORS Diep DB, Johnsborg O, Risoen PA, Nes IF.
TITLE Evidence for dual functionality of the operon plnABCD in the
regulation of the operon plnABCD in the regulation of bacteriocin
production in Lactobacillus plantarum
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7828)
AUTHORS Skaugen M, Axelsson L.
TITLE pELS200 E.coli/LActibacillus shuttle vector
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 7828)
AUTHORS Skaugen M, Axelsson L.
TITLE Direct Submission
JOURNAL Submitted (25-FEB-2004) Dept. of Chemistry, Biotechnology, and Food
Science, Agricultural University of Norway, Chr. Magnus Falsens vei
1, Aas N-1432, Norway
REFERENCE 4 (bases 1 to 7828)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 7828)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(25-FEB-2004) Dept. of Chemistry, Biotechnology, and Food Science,
Agricultural University of Norway, Chr. Magnus Falsens vei 1, Aas
N-1432, Norway"
COMMENT SGRef: number: 4; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7828
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 190..438
/gene="parD"
/label=parD
/note="Antitoxin ParD from Escherichia coli. Accession#:
P22995"
CDS 438..746
/gene="parE"
/label=parE
/note="Toxin ParE from Escherichia coli. Accession#:
Q79EC5"
primer_bind 883..899
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(1066..1800)
/gene="ermBP"
/label=ermBP
/note="rRNA adenine N-6-methyltransferase from Enterococcus
faecalis. Accession#: P0A4D5"
CDS complement(1897..1980)
/codon_start=1
/gene="ermBL"
/product="ErmBL"
/label=ermBL
/note="regulatory protein"
/protein_id="AAS77684.1"
/translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
gene complement(1897..1980)
/gene="ermBL"
/label=ermBL
oriT complement(3275..3383)
/direction=LEFT
/label=oriT
/note="incP origin of transfer"
promoter 3560..3664
/label=AmpR promoter
CDS 3665..4522
/label=AmpR
/note="beta-lactamase"
rep_origin 4772..5302
/label=oriV
/note="incP origin of replication"
regulatory 5524..5642
/note="Pneo; neomycin phosphotransferase promoter"
/regulatory_class="promoter"
CDS 5694..6839
/label=trfA
/note="trans-acting replication protein that binds to and
activates oriV"
primer_bind 7271..7287
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 7297..7315
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature complement(7324..7431)
/label=MCS
/note="pBluescript multiple cloning site"
promoter complement(7444..7462)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(7483..7499)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(7507..7523)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7531..7561)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(7576..7597)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
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