pELS100 vector (V007578)

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LOCUS       V007578                 7022 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007578
VERSION     V007578
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Rawlinson EL, Nes IF, Skaugen M.
  TITLE     LasX, a transcriptional regulator of the lactocin S biosynthetic
            genes in Lactobacillus sakei L45, acts both as an activator and a
            repressor
  JOURNAL   Biochimie 84 (5-6), 559-567 (2002)
   PUBMED   12423800
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Sorvig E, Skaugen M, Naterstad K, Eijsink VG, Axelsson L.
  TITLE     Plasmid p256 from Lactobacillus plantarum represents a new type of
            replicon in lactic acid bacteria, and contains a
            toxin-antitoxin-like plasmid maintenance system
  JOURNAL   Microbiology (Reading, Engl.) 151 (PT 2), 421-431 (2005)
   PUBMED   15699191
REFERENCE   3  (bases 1 to 7022)
  AUTHORS   Skaugen M, Axelsson L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2004) Dept. of Chemistry, Biotechnology, and Food
            Science, Agricultural University of Norway, Chr. Magnus Falsens vei
            1, Aas N-1432, Norway
REFERENCE   4  (bases 1 to 7022)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7022)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochimie
            84 (5-6), 559-567 (2002)"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Microbiology (Reading, Engl.) 151 (PT 2), 421-431 (2005)"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (25-FEB-2004) Dept. of Chemistry, Biotechnology, and Food Science,
            Agricultural University of Norway, Chr. Magnus Falsens vei 1, Aas
            N-1432, Norway"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     77..93
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(260..994)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     CDS             complement(1091..1174)
                     /codon_start=1
                     /gene="ermBL"
                     /product="ErmBL"
                     /label="ermBL"
                     /note="regulatory protein"
                     /protein_id="AAS77677.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            complement(1091..1174)
                     /gene="ermBL"
                     /label="ermBL"
     misc_feature    1315..2217
                     /note="p256 replication region; allows replication in
                     Lactobacillus species"
     oriT            complement(2469..2577)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     promoter        2754..2858
                     /label="AmpR promoter"
     CDS             2859..3716
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3966..4496
                     /label="oriV"
                     /note="incP origin of replication"
     regulatory      4718..4836
                     /note="Pneo; neomycin phosphotransferase promoter"
                     /regulatory_class="promoter"
     CDS             4888..6033
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     primer_bind     6465..6481
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6491..6509
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(6518..6625)
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(6638..6656)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6677..6693)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6701..6717)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6725..6755)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6770..6791)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."