Basic Vector Information
- Vector Name:
- pELS100
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7022 bp
- Type:
- Shuttle vector
- Replication origin:
- oriV
- Source/Author:
- Rawlinson EL, Nes IF, Skaugen M.
pELS100 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pELS100 vector Sequence
LOCUS 40924_17339 7022 bp DNA circular SYN 17-DEC-2018
DEFINITION Shuttle vector pELS100, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7022)
AUTHORS Rawlinson EL, Nes IF, Skaugen M.
TITLE LasX, a transcriptional regulator of the lactocin S biosynthetic
genes in Lactobacillus sakei L45, acts both as an activator and a
repressor
JOURNAL Biochimie 84 (5-6), 559-567 (2002)
PUBMED 12423800
REFERENCE 2 (bases 1 to 7022)
AUTHORS Sorvig E, Skaugen M, Naterstad K, Eijsink VG, Axelsson L.
TITLE Plasmid p256 from Lactobacillus plantarum represents a new type of
replicon in lactic acid bacteria, and contains a
toxin-antitoxin-like plasmid maintenance system
JOURNAL Microbiology (Reading, Engl.) 151 (PT 2), 421-431 (2005)
PUBMED 15699191
REFERENCE 3 (bases 1 to 7022)
AUTHORS Skaugen M, Axelsson L.
TITLE Direct Submission
JOURNAL Submitted (25-FEB-2004) Dept. of Chemistry, Biotechnology, and Food
Science, Agricultural University of Norway, Chr. Magnus Falsens vei
1, Aas N-1432, Norway
REFERENCE 4 (bases 1 to 7022)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 7022)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Biochimie
84 (5-6), 559-567 (2002)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName:
"Microbiology (Reading, Engl.) 151 (PT 2), 421-431 (2005)"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(25-FEB-2004) Dept. of Chemistry, Biotechnology, and Food Science,
Agricultural University of Norway, Chr. Magnus Falsens vei 1, Aas
N-1432, Norway"
COMMENT SGRef: number: 4; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7022
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 77..93
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(260..994)
/gene="ermBP"
/label=ermBP
/note="rRNA adenine N-6-methyltransferase from Enterococcus
faecalis. Accession#: P0A4D5"
CDS complement(1091..1174)
/codon_start=1
/gene="ermBL"
/product="ErmBL"
/label=ermBL
/note="regulatory protein"
/protein_id="AAS77677.1"
/translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
gene complement(1091..1174)
/gene="ermBL"
/label=ermBL
misc_feature 1315..2217
/note="p256 replication region; allows replication in
Lactobacillus species"
oriT complement(2469..2577)
/direction=LEFT
/label=oriT
/note="incP origin of transfer"
promoter 2754..2858
/label=AmpR promoter
CDS 2859..3716
/label=AmpR
/note="beta-lactamase"
rep_origin 3966..4496
/label=oriV
/note="incP origin of replication"
regulatory 4718..4836
/note="Pneo; neomycin phosphotransferase promoter"
/regulatory_class="promoter"
CDS 4888..6033
/label=trfA
/note="trans-acting replication protein that binds to and
activates oriV"
primer_bind 6465..6481
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 6491..6509
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature complement(6518..6625)
/label=MCS
/note="pBluescript multiple cloning site"
promoter complement(6638..6656)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(6677..6693)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(6701..6717)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6725..6755)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(6770..6791)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
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