Basic Vector Information
- Vector Name:
- pELI3.1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3892 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Yero CD, Pajon FR, Caballero ME, Cobas AK, Lopez HY, Farinas MM, Gonzales BS, Acosta DA.
- Promoter:
- CMV
pELI3.1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pELI3.1 vector Sequence
LOCUS 40924_17329 3892 bp DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector pELI3.1, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3892)
AUTHORS Yero CD, Pajon FR, Caballero ME, Cobas AK, Lopez HY, Farinas MM,
Gonzales BS, Acosta DA.
TITLE Immunization of mice with Neisseria meningitidis serogroup B genomic
expression libraries elicits functional antibodies and reduces the
level of bacteremia in an infant rat infection model
JOURNAL Vaccine 23 (7), 932-939 (2005)
PUBMED 15603895
REFERENCE 2 (bases 1 to 3892)
AUTHORS Yero D, Pajon R, Caballero E, Cobas K, Lopez Y, Farinas M, Gonzales
S, Acosta A.
TITLE Direct Submission
JOURNAL Submitted (19-JUL-2007) Molecular Biology, Finlay Institute, Ave 27,
Havana City, Havana 11600, Cuba
REFERENCE 3 (bases 1 to 3892)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3892)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Vaccine";
date: "2005"; volume: "23"; issue: "7"; pages: "932-939"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(19-JUL-2007) Molecular Biology, Finlay Institute, Ave 27, Havana
City, Havana 11600, Cuba"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3892
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 139..517
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 518..729
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 890..1022
/label=chimeric intron
/note="chimera between introns from human beta-globin and
immunoglobulin heavy chain genes"
promoter 1067..1085
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 1177..1193
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
regulatory 1207..1216
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 1242..1313
/label=multiple cloning site
/note="multiple cloning site"
primer_bind complement(1314..1330)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(1538..1555)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
polyA_signal complement(1573..1694)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 2091..2195
/label=AmpR promoter
CDS 2196..3053
/label=AmpR
/note="beta-lactamase"
rep_origin 3227..3815
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
This page is informational only.