Basic Vector Information
- Vector Name:
- pEGFP-TYR
- Antibiotic Resistance:
- Kanamycin
- Length:
- 6424 bp
- Type:
- Mammalian Expression
- Replication origin:
- ori
- Selection Marker:
- Neomycin (select with G418)
- Copy Number:
- High Copy
- Promoter:
- CMV
- Cloning Method:
- Restriction Enzyme
- 5' Primer:
- CMV forward
- 3' Primer:
- EGFP_N
pEGFP-TYR vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pEGFP-TYR vector Sequence
LOCUS V007014 6424 bp DNA circular SYN 13-MAY-2021 DEFINITION Exported. ACCESSION V007014 VERSION V007014 KEYWORDS pEGFP-TYR. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 6424) AUTHORS Halaban R, Svedine S, Cheng E, Smicun Y, Aron R, Hebert DN TITLE Endoplasmic reticulum retention is a common defect associated with tyrosinase-negative albinism. JOURNAL Proc Natl Acad Sci U S A. 2000 May 23;97(11):5889-94. PUBMED 10823941 REFERENCE 2 (bases 1 to 6424) TITLE Direct Submission REFERENCE 3 (bases 1 to 6424) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl Acad Sci U S A."; date: "2000-05-23"; volume: "97(11)"; pages: "5889-94" SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..6424 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 61..364 /label="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" CDS 643..2229 /gene="TYR" /label="Tyrosinase" /note="Tyrosinase from Homo sapiens. Accession#: P14679" CDS 2370..3086 /label="EGFP" /note="enhanced GFP" polyA_signal 3212..3333 /label="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(3340..3795) /direction=LEFT /label="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3822..3926 /label="AmpR promoter" promoter 3928..4285 /label="SV40 promoter" /note="SV40 enhancer and early promoter" CDS 4320..5111 /label="NeoR/KanR" /note="aminoglycoside phosphotransferase" primer_bind complement(5302..5321) /label="TK-pA-R" /note="Thymidine kinase polyA, reverse primer" polyA_signal 5346..5393 /label="HSV TK poly(A) signal" /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5722..6310 /direction=RIGHT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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