Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V007098 | pDGM6 | In stock, 1 week for quality controls |
Buy one, get one free! |
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.
Basic Vector Information
- Vector Name:
- pDGM6
- Antibiotic Resistance:
- Ampicillin
- Length:
- 21829 bp
- Type:
- AAV
- Replication origin:
- ori
- Copy Number:
- High Copy
- Cloning Method:
- Restriction Enzyme
pDGM6 vector Map
Plasmid Protocol
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5. Store the plasmid at -20 ℃.
6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it
General Plasmid Transform Protocol
1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.
2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.
3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.
4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.
5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.
pDGM6 vector Sequence
LOCUS V007098 21829 bp DNA circular SYN 13-JUL-2021
DEFINITION Exported.
ACCESSION V007098
VERSION V007098
KEYWORDS pDGM6
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 21829)
AUTHORS Gregorevic P, Blankinship MJ, Allen JM, Crawford RW, Meuse L, Miller
DG, Russell DW, Chamberlain JS
TITLE Systemic delivery of genes to striated muscles using
adeno-associated viral vectors.
JOURNAL Nat Med. 2004 Aug;10(8):828-34. doi: 10.1038/nm1085. Epub 2004 Jul
25.
PUBMED 15273747
REFERENCE 2 (bases 1 to 21829)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 21829)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nm1085";
journalName: "Nat Med"; date: "2004-08"; volume: "10"; issue: "8";
pages: "828-34"
SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..21829
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 21..123
/label="ITR"
/note="inverted terminal repeat of human adenovirus
serotype 5"
misc_signal 211..361
/label="Ad5 Psi"
/note="packaging signal for adenovirus serotype 5"
primer_bind 483..499
/label="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 483..499
/label="pBluescriptSK"
/note="For pBluescript vector"
CDS 1334..3196
/gene="Rep78"
/label="Protein Rep78"
/note="Protein Rep78 from Adeno-associated virus 2 (isolate
Srivastava/1982). Accession#: Q89268"
CDS 5268..5279
/label="WELQut site"
/note="WELQut protease recognition and cleavage site"
CDS 7507..8118
/gene="L3"
/label="Protease"
/note="Protease from Human adenovirus C serotype 5.
Accession#: P03253"
CDS complement(8220..9806)
/gene="DBP"
/label="DNA-binding protein"
/note="DNA-binding protein from Human adenovirus C serotype
5. Accession#: P03265"
CDS 9835..12255
/gene="L4"
/label="Shutoff protein"
/note="Shutoff protein from Human adenovirus C serotype 5.
Accession#: P24933"
CDS 12942..13622
/gene="L4"
/label="Pre-hexon-linking protein VIII"
/note="Pre-hexon-linking protein VIII from Human adenovirus
C serotype 5. Accession#: P24936"
CDS 14932..16674
/note="Fiber protein from Human adenovirus C serotype 5.
Accession#: P11818"
/label="Fiber protein"
CDS complement(17086..17967)
/note="Early 4 ORF6 protein from Human adenovirus C
serotype 2. Accession#: P03239"
/label="Early 4 ORF6 protein"
CDS complement(18246..18593)
/note="Early 4 ORF3 protein from Human adenovirus C
serotype 2. Accession#: P03241"
/label="Early 4 ORF3 protein"
CDS complement(19033..19416)
/note="Early 4 ORF1 protein from Human adenovirus C
serotype 2. Accession#: P03242"
/label="Early 4 ORF1 protein"
repeat_region 19726..19828
/label="ITR"
/note="inverted terminal repeat of human adenovirus
serotype 5"
rep_origin complement(20019..20607)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(20762..21619)
/label="AmpR"
/note="beta-lactamase"
promoter complement(21620..21724)
/label="AmpR promoter"
primer_bind 21792..21810
/label="pBRforEco"
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"