pCRB12i vector (V008186)

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LOCUS       40924_13335        5536 bp DNA     circular SYN 17-DEC-2018
DEFINITION  C.glutamicum-E.coli shuttle vector pCRB12i, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Oide S, Gunji W, Moteki Y, Yamamoto S, Suda M, Jojima T, Yukawa H, 
            Inui M.
  TITLE     Thermal and Solvent Stress Cross-Tolerance Conferred to 
            Corynebacterium glutamicum by Adaptive Laboratory Evolution
  JOURNAL   Appl. Environ. Microbiol. 81 (7), 2284-2298 (2015)
  PUBMED    25595768
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Gunji W, Oide S, Inui M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2015) Microbiology Group, Research Institute of 
            Innovative Technology for the Earth, 9-2 Kizugawadai, Kyoto 
            619-0292, Japan
REFERENCE   3  (bases 1 to 5536)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5536)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2015"; volume: "81"; issue: "7"; pages:
            "2284-2298"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-JAN-2015) Microbiology Group, Research Institute of Innovative 
            Technology for the Earth, 9-2 Kizugawadai, Kyoto 619-0292, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             71..1150
                     /label=lacI
                     /note="lac repressor"
     protein_bind    1166..1187
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      1621..2209
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             2757..3569
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    3635..5528
                     /note="derived from Corynebacterium glutamicum plasmid
                     pCG1"