pCR702 vector (V008193)

LOCUS       40924_13305        4937 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cre-lox Univector acceptor vector pCR702, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Jia F, Gampala SS, Mittal A, Luo Q, Rock CD.
  TITLE     Cre-lox univector acceptor vectors for functional screening in 
            protoplasts: analysis of Arabidopsis donor cDNAs encoding ABSCISIC 
            ACID INSENSITIVE1-like protein phosphatases
  JOURNAL   Plant Mol. Biol. 70 (6), 693-708 (2009)
  PUBMED    19499346
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Rock CD, Jia F, Gampala SSL., Mittal A, Luo Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2009) Biological Science, Texas Tech University, 
            2500 Broadway, Lubbock, TX 79409, USA
REFERENCE   3  (bases 1 to 4937)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4937)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2009"; volume: "70"; issue: "6"; pages: "693-708"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-FEB-2009) Biological Science, Texas Tech University, 2500 
            Broadway, Lubbock, TX 79409, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        411..2402
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             2430..2447
                     /label=6xHis
                     /note="6xHis affinity tag"
     protein_bind    complement(2448..2481)
                     /label=loxH
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021). The loxH variant 
                     was optimized for expression constructs but remains 
                     compatible with loxP."
     regulatory      2482..2701
                     /label=Cauliflower mosaic virus (CaMV) 35S terminator
                     /note="Cauliflower mosaic virus (CaMV) 35S terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2716..2732)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2740..2756)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2764..2794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2809..2830)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3118..3706)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3880..4737)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4738..4842)
                     /label=AmpR promoter