pCR3.1mBCL-XL vector (V008200)

Download GeneBank File(.gb)

LOCUS       V008200                 5771 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008200
VERSION     V008200
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Pirtskhalaishvili G, Shurin GV, Gambotto A, Esche C, Wahl M,
            Yurkovetsky ZR, Robbins PD, Shurin MR.
  TITLE     Transduction of dendritic cells with Bcl-xL increases their
            resistance to prostate cancer-induced apoptosis and antitumor effect
            in mice
  JOURNAL   J. Immunol. 165 (4), 1956-1964 (2000)
   PUBMED   10925278
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Gambotto A, Pagliano O, Shurin M, Robbins PD.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-1998) Vector Core Facility, University of
            Pittsburgh, 300 Technology Drive, Pittsburgh, PA 15219, USA
REFERENCE   3  (bases 1 to 5771)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5771)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Immunol."; date: "2000"; volume: "165"; issue: "4"; pages:
            "1956-1964"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-APR-1998) Vector Core Facility, University of Pittsburgh, 300
            Technology Drive, Pittsburgh, PA 15219, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        638..656
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             747..1445
                     /gene="Bcl2l1"
                     /label="Bcl-2-like protein 1"
                     /note="Bcl-2-like protein 1 from Mus musculus. Accession#:
                     Q64373"
     polyA_signal    1530..1754
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1886..2474)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     polyA_signal    complement(2803..2850)
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(3085..3870)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(3905..4262)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             complement(4325..5182)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5183..5287)
                     /label="AmpR promoter"
     rep_origin      5314..5769
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"