pCR-Zeo vector (V008210)

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LOCUS       40924_13215        5162 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pCR-Zeo, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Yan MY, Yan HQ, Ren GX, Zhao JP, Guo XP, Sun YC.
  TITLE     CRISPR-Cas12a-assisted recombineering in bacteria
  JOURNAL   Appl. Environ. Microbiol. (2017) In press
  PUBMED    28646112
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Yan M-Y., Yan H-Q., Ren G-X., Zhao J-P., Guo X-P., Sun Y-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2017) Moh Key Laboratory of Systems Biology of 
            Pathogens, Chinese Academy of Medical Sciences and Peking Union 
            Medical College Institute of Pathogen Biology, 9, Dongdan Santiao, 
            Dongcheng District, Beijing 100730, China
REFERENCE   3  (bases 1 to 5162)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5162)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-JUN-2017) Moh Key Laboratory of Systems Biology of Pathogens, 
            Chinese Academy of Medical Sciences and Peking Union Medical College
            Institute of Pathogen Biology, 9, Dongdan Santiao, Dongcheng 
            District, Beijing 100730, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      564..610
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     regulatory      747..903
                     /label=pHsp70
                     /note="pHsp70"
                     /regulatory_class="promoter"
     CDS             904..1275
                     /label=BleoR
                     /note="antibiotic-binding protein"
     rep_origin      1465..4218
                     /gene="PAL5000ts"
                     /label=oriM
                     /note="oriM"
     gene            1465..4218
                     /gene="PAL5000ts"
                     /label=PAL5000ts
     rep_origin      4357..4945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"