pCPC61 vector (V008239)

LOCUS       V008239                 5008 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008239
VERSION     V008239
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5008)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5008)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(195..1052)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1053..1157)
                     /label="AmpR promoter"
     CDS             1272..1298
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1302..1328
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1332..1358
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             2871..3989
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     protein_bind    complement(4242..4275)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(join(4441..5008,1..21))
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"