pCPC52 vector (V008243)

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LOCUS       V008243                12139 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008243
VERSION     V008243
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12139)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Fast and convenient doxycycline-induced marker recycling in Candida
            albicans via Cre-loxP system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12139)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 12139)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12139)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             262..1053
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             1263..1634
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     rep_origin      1775..2363
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2651..2672
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2687..2717
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2725..2741
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2749..2765
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2783..2801
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    2881..4839
                     /label="5' region of CdARG4 gene"
                     /note="5' region of CdARG4 gene"
     regulatory      4855..5589
                     /label="ADH1 promoter from Candida albicans SC5314"
                     /note="ADH1 promoter from Candida albicans SC5314"
                     /regulatory_class="promoter"
     CDS             5590..6207
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     CDS             6229..6570
                     /label="GAL4 activation domain"
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     regulatory      6588..6894
                     /label="ACT1 terminator from Candida albicans SC5314"
                     /note="ACT1 terminator from Candida albicans SC5314"
                     /regulatory_class="terminator"
     CDS             7356..8249
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Candida albicans
                     (strain SC5314 / ATCC MYA-2876). Accession#: P46586"
     protein_bind    8628..8898
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     CDS             9297..10325
                     /label="Cre"
                     /note="site-specific recombinase"
     misc_feature    10535..11500
                     /label="3' region of ARG4 gene"
                     /note="3' region of ARG4 gene"
     promoter        complement(11571..11589)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11596..11612)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             11750..12049
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"