pCPC51 vector (V008244)

LOCUS       40924_13025       11513 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCPC51, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11513)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Fast and convenient doxycycline-induced marker recycling in Candida 
            albicans via Cre-loxP system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11513)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 11513)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11513)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JAN-2018) Research Center of Bioenergy "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(365..383)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(390..406)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             544..843
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             1195..1986
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             2196..2567
                     /label=BleoR
                     /note="antibiotic-binding protein"
     rep_origin      2708..3296
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3603..3624
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3639..3669
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3677..3693
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3701..3717
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3735..3753
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    3875..5016
                     /label=5' region of HIS1 gene
                     /note="5' region of HIS1 gene"
     regulatory      5032..5766
                     /label=ADH1 promoter from Candida albicans SC5314
                     /note="ADH1 promoter from Candida albicans SC5314"
                     /regulatory_class="promoter"
     CDS             5767..6384
                     /label=TetR
                     /note="tetracycline repressor TetR"
     CDS             6406..6747
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     regulatory      6765..7071
                     /label=ACT1 terminator from Candida albicans SC5314
                     /note="ACT1 terminator from Candida albicans SC5314"
                     /regulatory_class="terminator"
     CDS             7418..8824
                     /codon_start=1
                     /product="argininosuccinate lyase Arg4"
                     /label=argininosuccinate lyase Arg4
                     /note="ARG4 gene from Candida albicans SC5314; ARG4"
                     /protein_id="AVR52680.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELHLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAVLK
                     QLENLKSILS"
     protein_bind    9180..9450
                     /label=tetracycline response element
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     CDS             9849..10877
                     /label=Cre
                     /note="site-specific recombinase"