pCotG-CL vector (V008276)

LOCUS       V008276                 6565 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008276
VERSION     V008276
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2013) Laboratory of Molecular Bacteriology,
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 6565)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6565)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-OCT-2013) Laboratory of Molecular Bacteriology, Medical
            University of Gdansk, Debinki 1, Gdansk 80-211, Poland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             378..1694
                     /gene="lysA"
                     /label="Diaminopimelate decarboxylase"
                     /note="Diaminopimelate decarboxylase from Bacillus subtilis
                     (strain 168). Accession#: P23630"
     misc_recomb     complement(1812..2198)
                     /label="pyrD"
                     /note="pyrD"
     promoter        2720..2824
                     /label="AmpR promoter"
     CDS             2825..3682
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3856..4444
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_recomb     complement(4672..5139)
                     /label="pryD"
                     /note="pryD"
     gene            5639..6389
                     /gene="cotG"
                     /label="cotG"
     regulatory      5639..5682
                     /gene="cotG"
                     /regulatory_class="promoter"
     regulatory      5713..5718
                     /gene="cotG"
                     /regulatory_class="ribosome_binding_site"
     CDS             5727..6389
                     /codon_start=1
                     /gene="cotG"
                     /product="CotG"
                     /label="cotG"
                     /note="spore coat protein"
                     /protein_id="AHG54616.1"
                     /translation="MGHYSHSDIEEAVKSAKKEGLKDYLYQEPHGKKRSHKKSHRTHKK
                     SRSHKKSYCSHKKSRSHKKSFCSHKKSRSHKKSYCSHKKSRSHKKSYRSHKKSRSYKKS
                     YRSYKKSRSYKKSCRSYKKSRSYKKSYCSHKKKSRSYKKSCRTHKKSYRSHKKYYKKPH
                     HHCDDYKRHDDYDSKKEYWKDGNCWVVKKKYKGSYGGGGSGGGGSGGGGSSGRACAS"