pCotC-CL vector (V008280)

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LOCUS       V008280                 5414 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008280
VERSION     V008280
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2013) Laboratory of Molecular Bacteriology,
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 5414)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5414)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-OCT-2013) Laboratory of Molecular Bacteriology, Medical
            University of Gdansk, Debinki 1, Gdansk 80-211, Poland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_recomb     complement(401..873)
                     /label="C-terminal part of lacA"
                     /note="C-terminal part of lacA"
     gene            896..1286
                     /gene="cotC"
                     /label="cotC"
     regulatory      896..1032
                     /gene="cotC"
                     /regulatory_class="promoter"
     regulatory      1040..1045
                     /gene="cotC"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    1053..1250
                     /gene="cotC"
                     /note="cotC; spore coat protein"
     misc_feature    1266..1286
                     /gene="cotC"
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     CDS             1494..2774
                     /gene="hisD"
                     /label="Histidinol dehydrogenase"
                     /note="Histidinol dehydrogenase from Bacillus subtilis
                     (strain 168). Accession#: O34651"
     misc_recomb     complement(2796..3174)
                     /label="N-terminal part of lacA"
                     /note="N-terminal part of lacA"
     primer_bind     complement(3193..3209)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3217..3233)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3241..3271)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3286..3307)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3595..4183)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4357..5214)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5215..5319)
                     /label="AmpR promoter"