pCon5 vector (V008291)

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LOCUS       V008291                 7082 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008291
VERSION     V008291
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Raja Abd Rahman RNZ., Wong CF, Salleh AB, Basri M.
  TITLE     Expression vector of pCon5
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7082)
  AUTHORS   Raja Abd Rahman RNZ., Wong CF, Salleh AB, Basri M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2011) Department of Microbiology, Universiti Putra
            Malaysia, Faculty of Biotechnology and Biomolecular Sciences, UPM
            Serdang, Selangor 43400, Malaysia
REFERENCE   3  (bases 1 to 7082)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7082)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-JAN-2011) Department of Microbiology, Universiti Putra Malaysia,
            Faculty of Biotechnology and Biomolecular Sciences, UPM Serdang,
            Selangor 43400, Malaysia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(62..650)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(824..1681)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1682..1786)
                     /label="AmpR promoter"
     promoter        1977..2054
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             2055..3134
                     /label="lacI"
                     /note="lac repressor"
     CDS             complement(3330..4823)
                     /gene="lasB"
                     /label="Elastase"
                     /note="Elastase from Pseudomonas aeruginosa (strain ATCC
                     15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C
                     / PRS 101 / PAO1). Accession#: P14756"
     misc_feature    complement(4869..4897)
                     /label="operator O3 of PT7(A1/O4/O3)"
                     /note="operator O3 of PT7(A1/O4/O3)"
     protein_bind    4879..4895
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    complement(4905..4910)
                     /label="promoter -10 of PT7(A1/O4/O3)"
                     /note="promoter -10 of PT7(A1/O4/O3)"
     protein_bind    complement(4912..4928)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    complement(4928..4933)
                     /label="promoter -33 of PT7(A1/O4/O3)"
                     /note="promoter -33 of PT7(A1/O4/O3)"
     rep_origin      complement(5662..6013)
                     /direction=LEFT
                     /label="pRO1600 oriV"
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             6027..6857
                     /label="pRO1600 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     primer_bind     7021..7037
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"