pColdI(SP-4) vector (V008315)

LOCUS       40924_12655        4407 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pColdI(SP-4) DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Suzuki M, Zhang J, Liu M, Woychik NA, Inouye M.
  TITLE     Single protein production in living cells facilitated by an mRNA 
            interferase
  JOURNAL   Mol. Cell 18 (2), 253-261 (2005)
  PUBMED    15837428
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Shodai T.
  TITLE     pColdI-IV(SP-4) vectors are derived from pColdI-IV(SP-2)[Ref.3], the
            ACA sequences within each transcription region were subsituted
  JOURNAL   Published Only in Database (2006)
REFERENCE   3  (bases 1 to 4407)
  AUTHORS   Shodai T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2006) Toshihiro Shodai, TAKARA BIO INC.; 
            Seta3-4-1, OTSU 520-2193, Japan (E-mail:shiyoudait@takara-bio.co.jp,
            Tel:81-77-543-7229, Fax:81-77-543-7238)
REFERENCE   4  (bases 1 to 4407)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4407)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Cell";
            date: "2005"; volume: "18"; issue: "2"; pages: "253-261"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Published 
            Only in Database (2006)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (01-FEB-2006) Toshihiro Shodai, TAKARA BIO INC."; volume: " 
            Seta3-4-1, OTSU 520-2193, Japan (E-mail:shiyoudait@takara-bio.co.jp,
            Tel:81-77-543-7229, Fax"; pages: "81-77-543-7238"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        15..81
                     /label=cspA promoter
                     /note="promoter of the E. coli cold shock protein cspA gene
                     (Mitta et al., 1997)"
     misc_feature    82..121
                     /gene="cspA"
                     /label=synthetic lac operator sequence
                     /note="synthetic lac operator sequence"
     5'UTR           122..255
                     /label=cspA 5'UTR
                     /note="5'UTR of the E. coli cold shock protein cspA gene
                     (Mitta et al., 1997)"
     CDS             256..270
                     /label=TEE
                     /note="translation enhancing element for E. coli (Qing et
                     al., 2004)"
     CDS             271..288
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             289..300
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     misc_feature    301..360
                     /gene="cspA"
                     /note="MCS = Multiple cloning sites contain the follow 
                     restriction sites: NdeI, SacI, KpnI, XhoI, BamHI, EcoRI, 
                     HindIII, SalI, PstI, XbaI"
     3'UTR           368..512
                     /label=cspA 3'UTR
                     /note="3'UTR of the E. coli cold shock protein cspA gene
                     (Mitta et al., 1997)"
     rep_origin      complement(711..1166)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1266..1370
                     /label=AmpR promoter
     CDS             1371..2228
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2402..2990
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    complement(3128..3149)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3165..4244)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(4245..4322)
                     /label=lacI promoter