Basic Vector Information
- Vector Name:
- pCMVTAG2b
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4324 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Lu Q.
- Promoter:
- CMV
pCMVTAG2b vector Map
pCMVTAG2b vector Sequence
LOCUS 40924_12365 4324 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector pCMVTAG2b, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4324) AUTHORS Lu Q. TITLE pCMVTAG2b JOURNAL Unpublished REFERENCE 2 (bases 1 to 4324) AUTHORS Lu Q. TITLE Direct Submission JOURNAL Submitted (19-JUN-1998) Technical Services, Stratagene, 11011 N. Torrey Pines Rd., San Diego, CA 92037, USA REFERENCE 3 (bases 1 to 4324) TITLE Direct Submission REFERENCE 4 (bases 1 to 4324) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (19-JUN-1998) Technical Services, Stratagene, 11011 N. Torrey Pines Rd., San Diego, CA 92037, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4324 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 67..370 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 371..574 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 620..638 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" regulatory 673..682 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" CDS 682..705 /label=FLAG /note="FLAG(R) epitope tag, followed by an enterokinase cleavage site" primer_bind complement(753..769) /label=KS primer /note="common sequencing primer, one of multiple similar variants" promoter complement(826..844) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 1118..1239 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(1246..1701) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 1728..1830 /label=AmpR promoter promoter 1834..2191 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 2226..3017 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" polyA_signal 3252..3299 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 3628..4216 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
This page is informational only.