Basic Vector Information
- Vector Name:
- pCMV-TALER24
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9605 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
pCMV-TALER24 vector Vector Map
pCMV-TALER24 vector Sequence
LOCUS 40924_11945 9605 bp DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector pCMV-TALER24, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9605)
AUTHORS Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
TITLE Modular construction of mammalian gene circuits using TALE
transcriptional repressors
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9605)
AUTHORS Li Y, Jiang Y, Chen H, Liao W.
TITLE Direct Submission
JOURNAL Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
REFERENCE 3 (bases 1 to 9605)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9605)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(03-SEP-2014) Bioinformatics Division/Center for Synthetic "
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..9605
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 1..21
/label=attB4
/note="core recombination site for the Gateway(R) BP
reaction"
enhancer 93..396
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 397..600
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
protein_bind 631..655
/label=attB1
/note="recombination site for the Gateway(R) BP reaction"
regulatory 664..673
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 673..714
/label=V5 tag
/note="epitope tag from simian virus 5"
CDS 727..747
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
misc_feature 757..3348
/label=similar to TALER24
/note="similar to TALER24"
protein_bind complement(3439..3463)
/label=attB2
/note="recombination site for the Gateway(R) BP reaction"
polyA_signal 3618..3673
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(4034..4050)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 4058..4074
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4082..4112)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4127..4148
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 4321..5157
/label=HA-R
/note="right homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
promoter complement(5187..5205)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(5226..5242)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(5250..5266)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5274..5304)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(5319..5340)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(5628..6216)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6390..7247)
/label=AmpR
/note="beta-lactamase"
promoter complement(7248..7352)
/label=AmpR promoter
rep_origin complement(7378..7833)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 7975..7991
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 8001..8019
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 8034..8837
/label=HA-L
/note="left homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
misc_feature 8916..8941
/label=SA
/note="splice acceptor site"
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