Basic Vector Information
- Vector Name:
- pCMV-TALER20
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10217 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
- Promoter:
- CMV
pCMV-TALER20 vector Vector Map
pCMV-TALER20 vector Sequence
LOCUS 40924_11925 10217 bp DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector pCMV-TALER20, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10217)
AUTHORS Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
TITLE Modular construction of mammalian gene circuits using TALE
transcriptional repressors
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 10217)
AUTHORS Li Y, Jiang Y, Chen H, Liao W.
TITLE Direct Submission
JOURNAL Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
REFERENCE 3 (bases 1 to 10217)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 10217)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(03-SEP-2014) Bioinformatics Division/Center for Synthetic "
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..10217
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 1..21
/label=attB4
/note="core recombination site for the Gateway(R) BP
reaction"
enhancer 93..396
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 397..600
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
protein_bind 631..655
/label=attB1
/note="recombination site for the Gateway(R) BP reaction"
regulatory 664..673
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 673..714
/label=V5 tag
/note="epitope tag from simian virus 5"
CDS 727..747
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
misc_feature 757..3960
/label=similar to TALER20
/note="similar to TALER20"
protein_bind complement(4051..4075)
/label=attB2
/note="recombination site for the Gateway(R) BP reaction"
polyA_signal 4230..4285
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(4646..4662)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 4670..4686
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4694..4724)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4739..4760
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 4933..5769
/label=HA-R
/note="right homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
promoter complement(5799..5817)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(5838..5854)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 5862..5878
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5886..5916)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(5931..5952)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(6240..6828)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7002..7859)
/label=AmpR
/note="beta-lactamase"
promoter complement(7860..7964)
/label=AmpR promoter
rep_origin complement(7990..8445)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 8587..8603
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 8613..8631
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 8646..9449
/label=HA-L
/note="left homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
misc_feature 9528..9553
/label=SA
/note="splice acceptor site"
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