pCMV-HIF-1a-FLAG vector (V008410)

LOCUS       V008410                 7858 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008410
VERSION     V008410
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Cook KM, Hilton ST, Mecinovic J, Motherwell WB, Figg WD, Schofield
            CJ.
  TITLE     Epidithiodiketopiperazines block the interaction between
            hypoxia-inducible factor-1alpha (HIF-1alpha) and p300 by a zinc
            ejection mechanism
  JOURNAL   J. Biol. Chem. 284 (39), 26831-26838 (2009)
   PUBMED   19589782
REFERENCE   2  (bases 1 to 7858)
  AUTHORS   Figg WD, Chau CH, Cook KM.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2009) NCI, NIH, 10 Center Drive, Bldg 10, Rm 5A01,
            Bethesda, MD 20892, USA
REFERENCE   3  (bases 1 to 7858)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7858)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biol.
            Chem."; date: "2009"; volume: "284"; issue: "39"; pages:
            "26831-26838"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2009) NCI, NIH, 10 Center Drive, Bldg 10, Rm 5A01, Bethesda,
            MD 20892, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     634..650
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        673..691
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     protein_bind    716..740
                     /gene="mutant version of attB"
                     /label="attB1"
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             743..3220
                     /gene="HIF1A"
                     /label="Hypoxia-inducible factor 1-alpha"
                     /note="Hypoxia-inducible factor 1-alpha from Homo sapiens.
                     Accession#: Q16665"
     protein_bind    complement(3219..3243)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3263..3286
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     polyA_signal    3616..3840
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3886..4314
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4328..4657
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4724..5515
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5582..5630
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     promoter        5936..6040
                     /label="AmpR promoter"
     CDS             6041..6898
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7072..7660
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"