pCM410.1145 vector (V008436)

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LOCUS       V008436                10125 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008436
VERSION     V008436
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10125)
  AUTHORS   Chou H-H., Berthet J, Marx CJ.
  TITLE     Growth rate modulates the selective advantage of recurrent
            transposition mutations arising from experimental populations grown
            in a metal-limited environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10125)
  AUTHORS   Marx CJ, Chou H-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2008) Organismic and Evolutionary Biology, Harvard
            University, 16 Divinity Avenue, Biolab 3079, Cambridge, MA 02138,
            USA
REFERENCE   3  (bases 1 to 10125)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10125)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-OCT-2008) Organismic and Evolutionary Biology, Harvard
            University, 16 Divinity Avenue, Biolab 3079, Cambridge, MA 02138,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..712
                     /label="oriV"
                     /note="incP origin of replication"
     rep_origin      1105..1693
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    1981..2002
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2017..2047
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2055..2071
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2079..2095
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      2206..2306
                     /label="PmxaF"
                     /note="PmxaF"
                     /regulatory_class="promoter"
     primer_bind     2446..2462
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(2475..2531)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     CDS             2622..3746
                     /gene="flhA"
                     /label="S-(hydroxymethyl)glutathione dehydrogenase"
                     /note="S-(hydroxymethyl)glutathione dehydrogenase from
                     Paracoccus denitrificans. Accession#: P45382"
     CDS             3792..4631
                     /codon_start=1
                     /gene="fghA"
                     /product="S-formylglutathione hydrolase"
                     /label="fghA"
                     /protein_id="ACR45022.1"
                     /translation="MTLAYETVSENRSFGGIQGVYRHQSQATGTPMTFAIYLPPDARHG
                     KVPVLWYLSGLTCTHENAMTKAGAQEWAAEYGIAVIFPDTSPRGEGVANDETYDLGQGA
                     GFYVDATEAPWAPHFRMWHYVTHELPELVFNNFPLDREAQGITGHSMGGHGALTIAMTF
                     PERYRSVSAFAPIAHPSESDWGRKQFAAYLGDDKAAWKRHDSTILMREKGYPGEVLIDQ
                     GASDQFLDLLKPEALAHAMAERRQPGTFRMQQGYDHSYFFVQSFMADHIRWHAERLG"
     gene            3792..4631
                     /gene="fghA"
                     /label="fghA"
     primer_bind     complement(4683..4699)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(5144..5785)
                     /label="TetR"
                     /note="tetracycline resistance regulatory protein"
     CDS             complement(6566..7378)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(8053..9198)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(9470..9841)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label="traJ"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(9874..9983)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"