pCLKSCLA25 vector (V008450)

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LOCUS       40924_11281       12186 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCLKSCLA25, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12186)
  AUTHORS   Ma BG, Duan XY, Niu JX, Ma C, Hao QN, Zhang LX, Zhang HP.
  TITLE     Expression of stilbene synthase gene in transgenic tomato using 
            salicylic acid-inducible Cre/loxP recombination system with 
            self-excision of selectable marker
  JOURNAL   Biotechnol. Lett. 31 (1), 163-169 (2009)
  PUBMED    18792793
REFERENCE   2  (bases 1 to 12186)
  AUTHORS   Ma BG, Duan XY, Niu JX, Zhang LX, Zhang HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-DEC-2007) Department of Horticulture, Agriculture 
            College, Shihezi University, Shihezi, Xin Jiang 832003, China
REFERENCE   3  (bases 1 to 12186)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12186)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Lett."; date: "2009"; volume: "31"; issue: "1"; pages: "163-169"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-DEC-2007) Department of Horticulture, Agriculture College, 
            Shihezi University, Shihezi, Xin Jiang 832003, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1219..1845
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             2282..3346
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      3415..3609
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3953..4093
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4279..4867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4957..5748)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    6173..6197
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(6275..6449)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(6488..7165)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    7356..7377
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7392..7422
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7430..7446
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7454..7470
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7530..7563
                     /label=loxP site
                     /note="loxP site"
     protein_bind    7530..7563
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      7597..8922
                     /label=pathogenesis-related protein 1a (PR-1a) promoter
                     /note="pathogenesis-related protein 1a (PR-1a) promoter"
                     /regulatory_class="promoter"
     CDS             8990..10018
                     /label=Cre
                     /note="site-specific recombinase"
     gene            complement(10380..11736)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     protein_bind    complement(11791..11824)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(11862..11878)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    12081..12105
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"