pCLEAN-S167 vector (V008452)

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LOCUS       V008452                10480 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008452
VERSION     V008452
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10480)
  AUTHORS   Thole V, Worland B, Snape JW, Vain P.
  TITLE     The pCLEAN dual binary vector system for Agrobacterium-mediated
            plant transformation
  JOURNAL   Plant Physiol. 145 (4), 1211-1219 (2007)
   PUBMED   17932303
REFERENCE   2  (bases 1 to 10480)
  AUTHORS   Thole V, Vain P.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2007) Department of Crop Genetics, John Innes
            Centre, Colney Lane, Norwich NR4 7UH, UK
REFERENCE   3  (bases 1 to 10480)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10480)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages:
            "1211-1219"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-SEP-2007) Department of Crop Genetics, John Innes Centre, Colney
            Lane, Norwich NR4 7UH, UK"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(7..25)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(43..59)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(67..83)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(91..121)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(136..157)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        404..432
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             480..1667
                     /label="TcR"
                     /note="tetracycline efflux protein"
     CDS             2169..3314
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             4698..5666
                     /label="RepA"
                     /note="replication protein of plasmid pSa"
     CDS             6064..6711
                     /label="TetR"
                     /note="tetracycline resistance regulatory protein"
     oriT            7395..7504
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             7537..7905
                     /label="traJ"
                     /note="oriT-recognizing protein"
     rep_origin      8035..8746
                     /label="oriV"
                     /note="incP origin of replication"
     misc_feature    9246..9270
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    9271..9322
                     /note="Inner T-DNA region containing 52 nt-long multiple
                     cloning site with NotI, PacI, SfiI, BsiWI, ApaI, XhoI and
                     SalI restriction sites"
     misc_feature    9323..9347
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    9348..9371
                     /note="right border overdrive sequence (Peralta et al.
                     (1986) EMBO J 5: 1137-1142)"
     CDS             9585..10385
                     /gene="virG"
                     /label="Regulatory protein VirG"
                     /note="Regulatory protein VirG from Agrobacterium
                     tumefaciens (strain 15955). Accession#: P62722"