Basic Vector Information
- Vector Name:
- pCLEAN-S161
- Antibiotic Resistance:
- Tetracycline
- Length:
- 9407 bp
- Type:
- Binary vector
- Replication origin:
- oriV
- Source/Author:
- Thole V, Worland B, Snape JW, Vain P.
- Promoter:
- tet
pCLEAN-S161 vector Map
pCLEAN-S161 vector Sequence
LOCUS 40924_11031 9407 bp DNA circular SYN 17-DEC-2018 DEFINITION Binary vector pCLEAN-S161, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9407) AUTHORS Thole V, Worland B, Snape JW, Vain P. TITLE The pCLEAN dual binary vector system for Agrobacterium-mediated plant transformation JOURNAL Plant Physiol. 145 (4), 1211-1219 (2007) PUBMED 17932303 REFERENCE 2 (bases 1 to 9407) AUTHORS Thole V, Vain P. TITLE Direct Submission JOURNAL Submitted (30-SEP-2007) Department of Crop Genetics, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK REFERENCE 3 (bases 1 to 9407) TITLE Direct Submission REFERENCE 4 (bases 1 to 9407) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant Physiol."; date: "2007"; volume: "145"; issue: "4"; pages: "1211-1219" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (30-SEP-2007) Department of Crop Genetics, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..9407 /mol_type="other DNA" /organism="synthetic DNA construct" promoter complement(7..25) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(43..59) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(67..83) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(91..121) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(136..157) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 404..432 /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" CDS 480..1667 /label=TcR /note="tetracycline efflux protein" CDS 2169..3314 /label=trfA /note="trans-acting replication protein that binds to and activates oriV" CDS 4698..5666 /label=RepA /note="replication protein of plasmid pSa" CDS 6064..6711 /label=TetR /note="tetracycline resistance regulatory protein" oriT 7395..7504 /label=oriT /note="incP origin of transfer" CDS 7537..7905 /label=traJ /note="oriT-recognizing protein" rep_origin 8035..8746 /label=oriV /note="incP origin of replication" misc_feature 9246..9270 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" misc_feature 9271..9322 /note="inner T-DNA region containing 52 nt-long multiple cloning site with NotI, PacI, SfiI, BsiWI, ApaI, XhoI and SalI restriction sites" misc_feature 9323..9347 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" misc_feature 9348..9371 /note="right border overdrive sequence (Peralta et al. (1986) EMBO J 5: 1137-1142)"
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