pCL25 vector (V008468)

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LOCUS       V008468                 6624 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008468
VERSION     V008468
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Luong TT, Lee CY.
  TITLE     Improved single-copy integration vectors for Staphylococcus aureus
  JOURNAL   J. Microbiol. Methods 70 (1), 186-190 (2007)
   PUBMED   17512993
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   Luong TT, Lee CY.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2007) Microbiology and Immunology, Univ of
            Arkansas for Medical Sciences, 4301 W. Markham St. Mail Slot 511,
            Little Rock, AR 72205, USA
REFERENCE   3  (bases 1 to 6624)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6624)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Microbiol. Methods"; date: "2007"; volume: "70"; issue: "1"; pages:
            "186-190"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-APR-2007) Microbiology and Immunology, Univ of Arkansas for
            Medical Sciences, 4301 W. Markham St. Mail Slot 511, Little Rock, AR
            72205, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     CDS             complement(715..2091)
                     /gene="tet"
                     /label="Tetracycline resistance protein"
                     /note="Tetracycline resistance protein from Staphylococcus
                     aureus. Accession#: P02983"
     CDS             complement(2883..3671)
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     CDS             complement(4926..5873)
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     rep_origin      complement(5921..6143)
                     /direction=LEFT
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"