pCL0160 vector (V008470)

LOCUS       V008470                 7487 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008470
VERSION     V008470
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7487)
  AUTHORS   Bayne AC, Boltz D, Owen C, Betz Y, Maia G, Azadi P, Archer-Hartmann
            S, Zirkle R, Lippmeier JC.
  TITLE     Vaccination against Influenza with Recombinant Hemagglutinin
            Expressed by Schizochytrium sp. Confers Protective Immunity
  JOURNAL   PLoS ONE 8 (4), E61790 (2013)
   PUBMED   23626728
REFERENCE   2  (bases 1 to 7487)
  AUTHORS   Bayne A-C.V., Lippmeier JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2012) Molecular Biology, DSM Nutritional Products,
            6480 Dobbin Rd., Columbia, MD 21045, USA
REFERENCE   3  (bases 1 to 7487)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7487)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2013"; volume: "8"; issue: "4"; pages: "E61790"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-NOV-2012) Molecular Biology, DSM Nutritional Products, 6480
            Dobbin Rd., Columbia, MD 21045, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..1063
                     /label="elongation factor 1 promoter"
                     /note="elongation factor 1 promoter"
                     /regulatory_class="promoter"
     CDS             1070..2773
                     /gene="HA"
                     /label="Hemagglutinin"
                     /note="Hemagglutinin from Influenza A virus (strain
                     A/Chicken/Hong Kong/220/1997 H5N1 genotype Gs/Gd).
                     Accession#: O89746"
     regulatory      2796..3435
                     /label="Pfa 3 terminator"
                     /note="Pfa 3 terminator"
                     /regulatory_class="terminator"
     polyA_signal    3578..3699
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3961..4549)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4723..5580)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5581..5685)
                     /label="AmpR promoter"
     regulatory      5970..6418
                     /label="alpha-tubulin promoter"
                     /note="alpha-tubulin promoter"
                     /regulatory_class="promoter"
     CDS             6422..7210
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    7343..7476
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"