pCK905 vector (V008481)

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LOCUS       V008481                 8172 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008481
VERSION     V008481
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8172)
  AUTHORS   Kraft C.
  TITLE     A toolbox for the analysis of weak and low abundant molecule
            interactions in vivo by M-Track in budding yeast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8172)
  AUTHORS   Brezovich A, Schuschnig M, Ammerer G, Kraft C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2014) Department of Biochemistry and Cell Biology,
            MFPL, University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria
REFERENCE   3  (bases 1 to 8172)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8172)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-AUG-2014) Department of Biochemistry and Cell Biology, MFPL,
            University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        135..239
                     /label="AmpR promoter"
     CDS             240..1097
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1271..1859
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2147..2168
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2183..2213
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2221..2237
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2245..2261
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2282..2300
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2987..4486
                     /gene="ATG18"
                     /label="Autophagy-related protein 18"
                     /note="Autophagy-related protein 18 from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P43601"
     CDS             4547..4567
                     /label="TEV site"
                     /note="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
     CDS             4598..4771
                     /label="ProtA"
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     CDS             5330..5419
                     /label="3xHA"
                     /note="three tandem HA epitope tags"
     terminator      5441..5688
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     promoter        complement(5707..5725)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5735..5751)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5892..6347
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(6481..7281)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(7282..7497)
                     /label="URA3 promoter"
     misc_feature    complement(join(7767..8172,1..98))
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"