Basic Vector Information
- Vector Name:
- pCK904
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7695 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Host:
- Yeast
- Source/Author:
- Kraft C.
- Promoter:
- URA3
pCK904 vector Map
pCK904 vector Sequence
LOCUS V008482 7695 bp DNA circular SYN 17-DEC-2018 DEFINITION Exported. ACCESSION V008482 VERSION V008482 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 7695) AUTHORS Kraft C. TITLE A toolbox for the analysis of weak and low abundant molecule interactions in vivo by M-Track in budding yeast JOURNAL Unpublished REFERENCE 2 (bases 1 to 7695) AUTHORS Brezovich A, Schuschnig M, Ammerer G, Kraft C. TITLE Direct Submission JOURNAL Submitted (28-AUG-2014) Department of Biochemistry and Cell Biology, MFPL, University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria REFERENCE 3 (bases 1 to 7695) TITLE Direct Submission REFERENCE 4 (bases 1 to 7695) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (28-AUG-2014) Department of Biochemistry and Cell Biology, MFPL, University of Vienna, Dr. Bohr Gasse 9, Vienna 1030, Austria" SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..7695 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 224..328 /label="AmpR promoter" CDS 329..1186 /label="AmpR" /note="beta-lactamase" rep_origin 1360..1948 /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2236..2257 /label="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." promoter 2272..2302 /label="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 2310..2326 /label="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2334..2350 /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" promoter 2371..2389 /label="T3 promoter" /note="promoter for bacteriophage T3 RNA polymerase" CDS 3076..4575 /gene="ATG18" /label="Autophagy-related protein 18" /note="Autophagy-related protein 18 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c). Accession#: P43601" CDS 4942..5031 /label="3xHA" /note="three tandem HA epitope tags" terminator 5053..5300 /label="CYC1 terminator" /note="transcription terminator for CYC1" promoter complement(5319..5337) /label="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(5347..5363) /label="M13 fwd" /note="common sequencing primer, one of multiple similar variants" rep_origin 5504..5959 /label="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" CDS complement(6093..6893) /label="URA3" /note="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" promoter complement(6894..7109) /label="URA3 promoter" misc_feature complement(join(7379..7695,1..187)) /label="CEN/ARS" /note="S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence"
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