pCK73 vector (V008488)

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LOCUS       40924_10856        5768 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCK73, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Kemmer C, Gitzinger M, Daoud-El Baba M, Djonov V, Stelling J, 
            Fussenegger M.
  TITLE     Self-sufficient control of urate homeostasis in mice by a synthetic 
            circuit
  JOURNAL   Nat. Biotechnol. 28 (4), 355-360 (2010)
  PUBMED    20351688
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Kemmer C, Gitzinger M, Daoud-El Baba M, Djonov V, Stelling J, 
            Fussenegger M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2010) Department of Biosystems Science and 
            Engineering, D-BSSE, ETH Zurich, Mattenstrasse 20, Basel 4058, 
            Switzerland
REFERENCE   3  (bases 1 to 5768)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5768)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Biotechnol."; date: "2010"; volume: "28"; issue: "4"; pages: 
            "355-360"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-NOV-2010) Department of Biosystems Science and Engineering, 
            D-BSSE, ETH Zurich, Mattenstrasse 20, Basel 4058, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        52..431
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        432..634
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     repeat_region   643..904
                     /label=synthetic octameric hucO repeat (hucO8)
                     /note="synthetic octameric hucO repeat (hucO8)"
     CDS             919..2427
                     /label=SEAP
                     /note="secreted alkaline phosphatase from human placenta"
     polyA_signal    complement(2485..2606)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      2709..2954
                     /label=simian virus 40 enhancer
                     /note="simian virus 40 enhancer"
                     /regulatory_class="enhancer"
     rep_origin      complement(3271..3859)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4033..4890)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4891..4995)
                     /label=AmpR promoter
     rep_origin      5022..5477
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5608..5656
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5670..5761
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"