pCK65 vector (V008489)

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LOCUS       V008489                 4828 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008489
VERSION     V008489
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Kemmer C, Gitzinger M, Daoud-El Baba M, Djonov V, Stelling J,
            Fussenegger M.
  TITLE     Self-sufficient control of urate homeostasis in mice by a synthetic
            circuit
  JOURNAL   Nat. Biotechnol. 28 (4), 355-360 (2010)
   PUBMED   20351688
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Kemmer C, Gitzinger M, Daoud-El Baba M, Djonov V, Stelling J,
            Fussenegger M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2010) Department of Biosystems Science and
            Engineering, D-BSSE, ETH Zurich, Mattenstrasse 20, Basel 4058,
            Switzerland
REFERENCE   3  (bases 1 to 4828)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4828)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
            Biotechnol."; date: "2010"; volume: "28"; issue: "4"; pages:
            "355-360"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-NOV-2010) Department of Biosystems Science and Engineering,
            D-BSSE, ETH Zurich, Mattenstrasse 20, Basel 4058, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..244
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     repeat_region   258..519
                     /label="synthetic octameric hucO repeat (hucO8)"
                     /note="synthetic octameric hucO repeat (hucO8)"
     sig_peptide     537..599
                     /label="Ig-kappa leader"
                     /note="leader sequence from mouse immunoglobulin kappa
                     light chain"
     CDS             600..1502
                     /gene="uaZ"
                     /label="Uricase"
                     /note="Uricase from Aspergillus flavus. Accession#: Q00511"
     polyA_signal    complement(1545..1666)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     regulatory      1769..2014
                     /label="simian virus 40 enhancer"
                     /note="simian virus 40 enhancer"
                     /regulatory_class="enhancer"
     rep_origin      complement(2331..2919)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3093..3950)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3951..4055)
                     /label="AmpR promoter"
     rep_origin      4082..4537
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4668..4716
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    4730..4821
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"