pCK302 vector (V008498)

LOCUS       40924_10806        5618 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Promoter vector pCK302, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Kelly CL, Liu Z, Yoshihara A, Jenkinson SF, Wormald MR, Otero JM, 
            Estevez AM, Kato A, Marqvorsen MH, Fleet GW, Estevez RJ, Izumori K, 
            Heap JT.
  TITLE     Synthetic chemical inducers and genetic decoupling enable orthogonal
            control of the rhaBAD promoter
  JOURNAL   ACS Synth Biol (2016) In press
  PUBMED    27247275
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Kelly CL, Liu Z, Yoshihara A, Jenkinson SF, Wormald MR, Otero J, 
            Estevez A, Kato A, Marqvorsen MHS., Fleet GWJ., Estevez RJ, Izumori 
            K, Heap JT.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2016) Centre for Synthetic Biology and Innovation,
            Department of Life Sciences, Imperial College London, South 
            Kensington Campus, London SW7 2AZ, United Kingdom
REFERENCE   3  (bases 1 to 5618)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5618)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-JAN-2016) Centre for Synthetic Biology and Innovation, 
            Department of Life Sciences, Imperial College London, South 
            Kensington Campus, London SW7 2AZ, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     875..891
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      908..1021
                     /label=rpn txn term terminator
                     /note="rpn txn term terminator"
                     /regulatory_class="terminator"
     regulatory      1028..1328
                     /label=bla txn term terminator
                     /note="bla txn term terminator"
                     /regulatory_class="terminator"
     promoter        1345..1462
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     CDS             1518..2231
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     CDS             2250..2267
                     /label=6xHis
                     /note="6xHis affinity tag"
     regulatory      2300..2474
                     /label=rrnB1 B2 T1 term terminator
                     /note="rrnB1 B2 T1 term terminator"
                     /regulatory_class="terminator"
     promoter        2561..2652
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      2583..2611
                     /label=Pamp promoter
                     /note="Pamp promoter"
                     /regulatory_class="promoter"
     CDS             2653..3510
                     /label=AmpR
                     /note="beta-lactamase"
     regulatory      3525..3558
                     /note="Translation initiation rate of 20000 using Salis RBS
                     calculator; MG1655 rhaS RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             3559..4392
                     /label=rhaS
                     /note="positive regulator of the rhaB promoter"
     CDS             4467..5546
                     /label=lacI
                     /note="lac repressor"