pCK146 vector (V008508)

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LOCUS       V008508                 5213 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008508
VERSION     V008508
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Kemmer C, Gitzinger M, Daoud-El Baba M, Djonov V, Stelling J,
            Fussenegger M.
  TITLE     Self-sufficient control of urate homeostasis in mice by a synthetic
            circuit
  JOURNAL   Nat. Biotechnol. 28 (4), 355-360 (2010)
   PUBMED   20351688
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Kemmer C, Gitzinger M, Daoud-El Baba M, Djonov V, Stelling J,
            Fussenegger M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2010) Department of Biosystems Science and
            Engineering, D-BSSE, ETH Zurich, Mattenstrasse 20, Basel 4058,
            Switzerland
REFERENCE   3  (bases 1 to 5213)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5213)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
            Biotechnol."; date: "2010"; volume: "28"; issue: "4"; pages:
            "355-360"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-NOV-2010) Department of Biosystems Science and Engineering,
            D-BSSE, ETH Zurich, Mattenstrasse 20, Basel 4058, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        52..431
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        432..634
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     repeat_region   643..904
                     /label="synthetic octameric hucO repeat (hucO8)"
                     /note="synthetic octameric hucO repeat (hucO8)"
     sig_peptide     922..984
                     /label="Ig-kappa leader"
                     /note="leader sequence from mouse immunoglobulin kappa
                     light chain"
     CDS             985..1887
                     /gene="uaZ"
                     /label="Uricase"
                     /note="Uricase from Aspergillus flavus. Accession#: Q00511"
     polyA_signal    complement(1930..2051)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     regulatory      2154..2399
                     /label="simian virus 40 enhancer"
                     /note="simian virus 40 enhancer"
                     /regulatory_class="enhancer"
     rep_origin      complement(2716..3304)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3478..4335)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4336..4440)
                     /label="AmpR promoter"
     rep_origin      4467..4922
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5053..5101
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    5115..5206
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"