pCIE vector (V008511)

LOCUS       V008511                 7548 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008511
VERSION     V008511
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7548)
  AUTHORS   Weaver KE, Chen Y, Miiller EM, Johnson JN, Dangler AA, Manias DA,
            Clem AM, Schjodt DJ, Dunny GM.
  TITLE     Examination of E. faecalis toxin-antitoxin (TA) toxin Fst function
            utilizing a pheromone-inducible expression vector with tight
            repression and large dynamic range
  JOURNAL   J. Bacteriol. (2017) In press
   PUBMED   28348028
REFERENCE   2  (bases 1 to 7548)
  AUTHORS   Weaver K, Chen Y, Miiller E, Johnson J, Dangler A, Manias D, Clem A,
            Schjodt D, Dunny G.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7548)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7548)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol. (2017) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-MAR-2017) Department of Microbiology "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7548
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(74..1027)
                     /codon_start=1
                     /gene="prgX"
                     /product="PrgX"
                     /label="prgX"
                     /protein_id="ARF06186.1"
                     /translation="MFKIGSVLKQIRQELNYHQIDLYSGIMSKSVYIKVEADSRPISVE
                     ELSKFSERLGVNFFEILNRAGMNTKSVNETGKEKLLISKIFTNPDLFDKNFQRIEPKRL
                     TSLQYFSIYLGYISIAHHYNIEVPTFNKTITSDLKHLYDKRTTFFGIDYEIVSNLLNVL
                     PYEEVSSIIKPMYPIVDSFGKDYDLTIQTVLKNALTISIMNRNLKEAQYYINQFEHLKT
                     IKNISINGYYDLEINYLKQIYQFLTDKNIDSYLNAVNIINIFKIIGKEDIHRSLVEELT
                     KISAKEKFTPPKEVTMYYENYVAIENNPIPEIKEQS"
     gene            complement(74..1027)
                     /gene="prgX"
                     /label="prgX"
     regulatory      1206..1214
                     /regulatory_class="promoter"
     CDS             1236..1307
                     /codon_start=1
                     /gene="prgQ"
                     /product="PrgQ"
                     /label="prgQ"
                     /protein_id="ARF06187.1"
                     /translation="MKTTLKKLSRYIAVVIAITLIFI"
     gene            1236..1307
                     /gene="prgQ"
                     /label="prgQ"
     regulatory      1556..1600
                     /regulatory_class="terminator"
     regulatory      1699..1749
                     /regulatory_class="terminator"
     CDS             complement(2152..2163)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             2625..3272
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(3619..4779)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B"
                     /label="repB"
                     /protein_id="ARF06189.1"
                     /translation="MSSISKNEPNQKQVQTLNELSKRKVVEHNSLITSIAKMDKTPLKM
                     FELAVSCINTEEPPKDHTVYLSKEELFAFFKVSDNDKHSRFKQAVENMQKQAFFQIKEE
                     VGKGFKFRSIVPIPYVEWTDYHDDVKIEFHREIMPYLINLKQNFTQHALSDIAELNSKY
                     SIILYRWLSMNYNQYEHYSYKGGRREEQVEAYRNPTISMRELREMTDTVDEYPRFDRLE
                     HRVLKEPIEEINENTSFNVTYDKIKKGRSIDSIVFHITKKRRADDNSYKLEDKDYQSDK
                     EEKSRNEADLLKQAMESKYTRLLIENFLLSPLEMTDTALMAGLQKNVYPLYDELKELRG
                     LNGVKDHLSYISSKKEAYSKRNVAKYLKKAIEQYLPTVKRQDLNHE"
     gene            complement(3619..4779)
                     /gene="repB"
                     /label="repB"
     misc_feature    6144..6284
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6470..7058)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"