pCHIKV-LR ic vector (V008521)

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LOCUS       V008521                13756 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008521
VERSION     V008521
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13756)
  AUTHORS   Tsetsarkin K, Higgs S, McGee CE, De Lamballerie X, Charrel RN,
            Vanlandingham DL.
  TITLE     Infectious clones of Chikungunya virus (La Reunion isolate) for
            vector competence studies
  JOURNAL   Vector Borne Zoonotic Dis. 6 (4), 325-337 (2006)
   PUBMED   17187566
REFERENCE   2  (bases 1 to 13756)
  AUTHORS   Tsetsarkin KA, Vanlandingham DL, Higgs S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2007) Pathology, University of Texas Medical
            Branch, University Blvd, Galveston, TX 77555-0609, USA
REFERENCE   3  (bases 1 to 13756)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13756)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Vector
            Borne Zoonotic Dis."; date: "2006"; volume: "6"; issue: "4"; pages:
            "325-337"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-OCT-2007) Pathology, University of Texas Medical Branch,
            University Blvd, Galveston, TX 77555-0609, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     5'UTR           1..76
     CDS             77..7498
                     /note="Polyprotein P1234 from Chikungunya virus (strain
                     S27-African prototype). Accession#: Q8JUX6"
                     /label="Polyprotein P1234"
     CDS             7567..11310
                     /note="Structural polyprotein from Chikungunya virus
                     (strain S27-African prototype). Accession#: Q8JUX5"
                     /label="Structural polyprotein"
     3'UTR           11314..11811
     polyA_site      11812
     promoter        11927..12031
                     /label="AmpR promoter"
     CDS             12032..12889
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      13063..13651
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        13739..13756
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"