Basic Vector Information
- Vector Name:
- pCHF3301
- Antibiotic Resistance:
- Kanamycin
- Length:
- 12806 bp
- Type:
- Plant binary expression vector
- Replication origin:
- ori
- Host:
- Plants
- Source/Author:
- Li JT, Yu G, Sun XH, Jia CG, Du Q, Li QY, Pan HY.
- Promoter:
- CaMV35S(long)
pCHF3301 vector Map
pCHF3301 vector Sequence
LOCUS 40924_10646 12806 bp DNA circular SYN 17-DEC-2018
DEFINITION Plant binary expression vector pCHF3301, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12806)
AUTHORS Li JT, Yu G, Sun XH, Jia CG, Du Q, Li QY, Pan HY.
TITLE Modification of vectors for functional genomic analysis in plants
JOURNAL Genet. Mol. Res. 13 (3), 7815-7825 (2014)
PUBMED 25299096
REFERENCE 2 (bases 1 to 12806)
AUTHORS Yu G, Sun X, Li J, Pan H.
TITLE Direct Submission
JOURNAL Submitted (07-JUN-2013) Plant Pathology, College of Plant Science,
Jilin University, Xi'an Road 5333, Changchun, Jilin 130062, China
REFERENCE 3 (bases 1 to 12806)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 12806)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Genet. Mol.
Res."; date: "2014"; volume: "13"; issue: "3"; pages: "7815-7825"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(07-JUN-2013) Plant Pathology, College of Plant Science, Jilin
University, Xi'an Road 5333, Changchun, Jilin 130062, China"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Assembly Method :: Lasergene v. 7.1
Sequencing Technology :: 454
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..12806
/mol_type="other DNA"
/organism="synthetic DNA construct"
intron 17..206
/label=cat1 intron
/note="castor bean catalase intron, modified"
CDS 201..2024
/codon_start=1
/label=GUS
/note="beta-glucuronidase"
/translation="LQNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQ
ESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWV
NNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQ
SYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELR
DADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLRVGIRSVAV
KGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAE
EMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKE
LIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHT
DTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAG
LHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIF
TRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
CDS 2031..2048
/codon_start=1
/label=6xHis
/note="6xHis affinity tag"
/translation="HHHHHH"
terminator 2083..2335
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 2357..2381
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 3681..4307
/codon_start=1
/label=pVS1 StaA
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 4744..5808
/codon_start=1
/label=pVS1 RepA
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
rep_origin 5877..6071
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 6415..6555
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(6741..7329)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7419..8210)
/codon_start=1
/label=KanR
/note="aminoglycoside phosphotransferase"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 8635..8659
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal complement(8737..8911)
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(8921..9469)
/codon_start=1
/label=BlpR
/note="phosphinothricin acetyltransferase"
/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
WQLDFSLPVPPRPVLPVTEI"
promoter complement(9514..10191)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
protein_bind 10382..10403
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 10418..10448
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 10456..10472
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 10480..10496
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 10519..11353
/label=CaMV35S(long)
/note="Cauliflower mosaic virus 35S promoter (long)"
terminator 11399..12040
/label=E9 terminator
/note="terminator and polyadenylation signal from the pea
rbcS-E9 gene"
primer_bind complement(12053..12069)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 12446..12791
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
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