pCHCC155 vector (V008528)

LOCUS       40924_10636        4956 bp DNA     circular SYN 17-DEC-2018
DEFINITION  BiFC vector pCHCC155, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Gong Z, Lu H.
  TITLE     A new combination of fluorescent protein fragments suitable for 
            green-colored bimolecular fluorescence complementation (BiFC) assays
            in Schizosaccharomyces pombe
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Gong Z, Lu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2012) School of Life Science, Fudan University, 
            Room 618, Science Building, Handan Road 220, Shanghai 200433, China
REFERENCE   3  (bases 1 to 4956)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4956)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-DEC-2012) School of Life Science, Fudan University, Room 618, 
            Science Building, Handan Road 220, Shanghai 200433, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        235..253
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    294..344
                     /label=linker
                     /note="linker"
     CDS             345..596
                     /label=VC155
                     /note="C-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
     CDS             597..614
                     /label=6xHis
                     /note="6xHis affinity tag"
     regulatory      624..1116
                     /label=Saccharomyces cerevisiae LEU2 terminator
                     /note="Saccharomyces cerevisiae LEU2 terminator"
                     /regulatory_class="terminator"
     gene            1162..2737
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin
                     resistance"
     promoter        complement(2845..2863)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3121..3709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3883..4740)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4741..4845)
                     /label=AmpR promoter