pCGTNG vector (V008531)

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LOCUS       40924_10616       13152 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gateway adapted binary vector pCGTNG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13152)
  AUTHORS   Zhang C, Galbraith DW.
  TITLE     A Gateway adapted Agrobacterium binary vector series for plant 
            biological studies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13152)
  AUTHORS   Zhang C, Galbraith DW.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2006) Department of Plant Sciences, University of 
            Arizona, 303 Forbes Building, Tucson, AZ 85721-0036, USA
REFERENCE   3  (bases 1 to 13152)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13152)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-JAN-2006) Department of Plant Sciences, University of Arizona, 
            303 Forbes Building, Tucson, AZ 85721-0036, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        75..744
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     5'UTR           759..893
                     /note="translation leader sequence; 5'UTR from tobacco etch
                     virus"
     protein_bind    901..1020
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1050..1080
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1134..1790
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2135..2437
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2481..2605)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2610..3326
                     /label=EGFP
                     /note="enhanced GFP"
     regulatory      3339..3550
                     /label=cauliflower mosaic virus 35S poly(A) signal
                     /note="cauliflower mosaic virus 35S poly(A) signal"
                     /regulatory_class="polyA_signal_sequence"
     primer_bind     complement(3572..3588)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      4181..4433
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4455..4479
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5779..6405
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             6842..7906
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      7975..8169
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8513..8653
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8839..9427)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9517..10308)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     repeat_region   10733..10758
                     /label=left border repeat from C58 T-DNA
                     /note="left border repeat from C58 T-DNA"
     misc_feature    10733..10757
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(10835..11009)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11052..12074)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(12142..12819)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    13010..13031
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        13046..13076
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13084..13100
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13108..13124
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"